Immobilization of Bioactive Peptides to Silane-Patterned Silicon
Silicon wafer substrates were patterned with OTS and DETA (St. John et. al. 1997). Synthetic peptides, either Arginine-Glycine-Aspartate-Serine (RGDS) or Lysine-Histidine-Isoleucine-Phenylalanine-Serine-Aspartate-Aspartate-Serine-Serine-Glutamate (KHIFSDDSSE), were then covalently attached to DETA-modified silicon using a carbodiimide reaction.
Selective Attachment of Astroglial Cells to Peptide Modified Silicon. Under serum-free conditions, LRM55 cells attached and spread on silicon modified with either DETA-RGDS or DETA-KHIFSDDSSE; these cells attached but did not spread only on silicon modified with either DETA or OTS. In contrast, primary astrocytes selectively attached and spread on silicon modified with DETA-KHIFSDDSSE. Cell membranes and OTS-modified silicon were stained with cyanine dye.
Last modified March 20 1998