We describe new methods for molecularly patterning surfaces to guide the selective attachment and growth of central nervous system cells in culture. Control of attachment and growth of neurons and glia is desirable for neurological implants and research on central nervous system function.
Molecular patterning of silicon surfaces with proteins, synthetic polypeptides, and silane based Self Assembled Monolayers (SAMS) was achieved by microcontact printing (µCP). New developments described will include "thin stamp µCP" for improved geometrical control of the pattern, optimization of the stamp master for printing isolated micrometer size features, and plasma treatment of the stamp for use with aqueous printing solutions. Using theses refinements, we have generated high quality patterned molecular layers with isolated features down to 1 µm. Fluorescence microscopy and Atomic Force Microscopy (AFM) were performed to determine the coverage and morphology of the patterned layers.
Figures below: (top,left) SEM of a µCP elastomer stamp. Scale bar = 100 µm. (top,right) SEM of a set of microelectrodes on our planar microelectrode array. (bottom,left) Neuronal cell network demonstrating bursting activity at the indicated electrode. The raw signal is shown on the right with an autocorrelogram below indicating a burst period of 4-5 seconds. (bottom,right) Cell network with weak activity at the indicated electrode.
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Last modified 7 April 1998
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