J. Han and H. G. Craighead
School of Applied and Engineering Physics, Cornell University
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Following is the video clips showing the motion of DNA molecules in our entropic sieving channel. Appropriate viewers like this should be installed in your computer to see these movies.
Depending on the browser you are using, the actual appearance of this page might be slihtly different. If no control buttons are showing, click the image to play each clip.
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Real time video of lambda phage DNA (48.5 kbps) motion in the entropic sieve channel.
The original 30 frame per second (fps) video signal was
digitized at 6 fps and contrast-enhanced by a computer. The brighter regions in the channel are the thick regions, which is
1.4 µm deep. Thin regions (darker regions) are 90 nm deep. The length of each
thick or thin regions is 10 µm. DNA is moving faster
in the thin region because electric field is higher. One can see the DNA deformation
and stretching when it enters a thin region.
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Motion of individual T2 (164 kbps) and T7 (37.9 kbps) DNA molecules in the channel.
(real time, frame rate : 3 fps, thick region : 650 nm, thin region : 90 nm)
The right channel
is 4 µm period channel, where one can see the larger T2 DNA is catching up
other four smaller T7 DNA. Compare the speed of the big one near the above edge
of the field, with that of other small T7 DNAs. The length of vertical field
of this image was 140 µm, and the average electric field was 45.5 V/cm.
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Last modified 17 March 1999